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Publication Details
Year :

2013

Journal :

Mohd Apandi Y, Zarina MZ, Khairul Azuan O, Nur Ismawati AR and Zainah S (2013). Molecular characterization of adenovirus causing acute respiratory disease in Malaysia from 2003 to 2011. Journal of General and Molecular Virology 5(2): 14-21

Abstract :

Human Adenoviruses (HAdVs) are human pathogens that normally are associated with acute respiratory disease (ARD). Here, in this study, we characterized human adenovirus isolated from respiratory specimens collected from 2003 to 2011. Positive adenovirus were confirmed by cell culture and indirect immunoflourescence (IF) techniques. The isolates and positive clinical samples were subjected to adenovirus polymerase chain reaction, followed by DNA sequencing. BLAST searches and phylogenetic analysis revealed that only 2 species of HAdV, species C at 64% (47/73) and species B at 36% (26/73) were found circulating amongst ARD cases in Malaysia. Four types of HAdV species C were detected namely: HAdV-2 (66%), HAdV-1(17%), HAdV-5(13%) and HAdV-6(4%). For HAdV species B, only 2 types were detected with HAdV-3 being the highest at 58% and HAdV-7 at 42%. HadV-7 was associated with severe infections.

myGOV MAMPU JPA IKU MSC NMMR CRC MyHEALTH MOH Globinmed SPA krste.my

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